Mucosal and systemic immune profiles differ during early and late phase of the disease in patients with active ulcerative colitis.
J Crohns Colitis. 2019 Apr 04;:
Authors: Mavroudis G, Magnusson MK, Isaksson S, Sundin J, Simrén M, Öhman L, Strid H
BACKGROUND AND AIMS: Alterations in the immunopathogenesis in ulcerative colitis (UC) during the disease course have been proposed. We therefore aimed to determine mucosal and systemic immune profiles in individual patients at the time of diagnosis (early disease) and after 10 years (late disease).
METHODS: Patients with UC provided serum and mucosal biopsies during a flare at early and late disease. Serum samples were analyzed using the Olink Proseek Inflammation panel. mRNA gene expression of biopsies was analyzed using the Qiagen RT2 Profiler PCR Arrays Antibacterial response and T Helper Cell Differentiation.
RESULTS: Orthogonal projections to latent structures discriminant analyses (OPLS-DA) demonstrated that the profile of 15 serum proteins discriminated early and late disease (R2=0.84, Q2=0.65) in 15 UC patients, 8 of these proteins were differently expressed between the groups (q<0.05). Further, OPLS-DA of the mRNA profiles in biopsies strongly discriminated early and late disease with high predictability (R2=0.96, Q2=0.89), 42 genes were differently expressed at the two time points (q<0.05). Finally, principal component analysis showed that T helper (Th) 1 and Th2 related genes were associated with early disease and late disease, respectively, and hierarchical cluster analysis was able to cluster patients with early from late disease with only minor overlap.
CONCLUSIONS: Mucosal and systemic immune profiles differ between early and late disease in patients with active UC, with a transition from a Th1 to a Th2 driven disease in the intestine. Improved understanding of the variation in immunopathogenesis during the disease course in UC is important to guide individualized treatment decision-making.
PMID: 30946450 [PubMed – as supplied by publisher]