Characterization of γδ T cells in intestinal mucosa from patients with early onset or long standing inflammatory bowel disease and their correlation with clinical status.
J Crohns Colitis. 2019 Jan 21;:
Authors: Lo Presti E, Di Mitri R, Mocciaro F, Di Stefano AB, Scibetta N, Unti E, Cicero G, Pecoraro G, Conte E, Dieli F, Meraviglia S
Background and aims: Inflammatory bowel disease is a complex chronic inflammatory disease of the human gut with no clear etiology. Traditionally, dysregulated adaptive immune responses play an important role even though accumulating evidence suggest a role also for innate immunity. Because of the well known plasticity of γδ T cells, we investigated their percentage, phenotypical features and effector functions in the intestinal mucosa of early onset and long standing IBD patients, as compared to healthy subjects.
Methods: Fresh biopsies from 30 CD and UC patients were obtained, digested and cells were analyzed by flow cytometry.
Results: We found a reduced frequency of Vδ1 T cells in tissue from early and late IBD patients (2.24% and 1.95% in early and late patients, respectively, versus 5.44% in healthy tissue) but an increased frequency of Vδ2 T cells in the gut of late IBD patients (3.19% in late patients versus 1.5% in early patients and 1.65% in healthy tissue). The infiltrating Vδ2 T cells had predominant effector memory and terminally-differentiated phenotypes and produced elevated levels of TNF-α and IL-17. The frequency of tissue Vδ2 T cells correlated with the extent of the inflammatory response and the severity of IBD.
Conclusion: Our study shows that tissue Vδ1 T cells are decreased in IBD patients while Vδ2 T cells are increased in the gut of IBD patients and contribute to TNF-α production. Moreover, we identify a yet unappreciated role of Vδ2 T cells in IL-17 production in the gut of long standing IBD patients suggesting that they also participate to the chronic inflammatory process.
PMID: 30689780 [PubMed – as supplied by publisher]